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Gladstone Institutes
About the role:
The Gladstone-UCSF Institute of Genomic Immunology is recruiting a Research Associate (RA) to help perform studies related to genetic engineering and functional assessment of T cell therapies, primarily CAR-T cells. Our institute has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells. These new technical abilities have furthered our research into the genetic control of T cell biology as well as opened the door to novel cancer immunotherapies based on engineered human immune cells. This individual would be part of a preclinical research team under the direction of Justin Eyquem (UCSF) streamlining the generation, benchmarking, and functional assessment of genetically engineered human T cell therapies for cancer and inflammatory disease.
What you will do:
The RA will utilize various protocols to generate the molecular components, such as DNA constructs, gRNA library, lentiviral vectors and Adeno-Associated Vectors necessary for specific cellular engineering experiments. Additional responsibilities will consist of isolation and culture of primary human T cells, as well as functional analysis of engineered cells via flow cytometry, cellular assays, Sanger sequencing, next-generation DNA sequencing, Single-Cell RNA sequencing. The technician’s role will also include the design and completion of cellular editing experiments, as well as in vitro analysis of novel cellular cancer immunotherapies and coordination with the in vivo team for mouse experiments employing the T cell products under development.
Strong organizational and communication skills are required.
Develop cell therapy candidates through in vitro and in vivo experiments
Cell engineering and culturing, including primary immune cells and cancer cells
Assist with tumor assessment, tissue dissection (e.g. spleen, bone marrow, lymph nodes, engrafted tumors) for downstream analysis
Cloning and other molecular biology tasks
Maintain and ensure safe laboratory environment and work practices.
Follow standard operating procedures (SOP) and IACUC protocols.
Work collaboratively across a dynamic scientific environment
What you will need:
BS or BA or MS 2+years related experience in cellular Biology, immunology, or related field; or an equivalent combination of education and experience
New graduates with a commitment to learning are welcome to apply
Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.)
Experience with T cell engineering using viral and non-viral gene editing technologies, including retroviral vectors, AAV, RNA/RNP electroporation and analysis via multicolor flow cytometry
Experience with in vitro characterization of engineered T cells to evaluate cytotoxicity, proliferation, cytokine production and phenotype
Experience with animal studies, including basic animal care, tumor monitor and assessment is a plus
Experience with library design, screening and NGS sequencing is a plus
Excellent attention to detail, organizational and interpersonal communication skills (verbal and written)
Willingness and ability to learn new methods and proactive at troubleshooting and optimization
Ability to work independently and as a member of a research team
Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines
Ability to comply with policies and procedures
Some evening and weekend hours are required
What Gladstone will offer you
People–work with talented, committed, and supportive teammates within an organization that values each member of its community.
A meaningful place to grow and learn–whether it is your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone’s mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow’s leaders in an inspiring and diverse environment.
Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
Compensation–competitive salary. Title and salary will be commensurate with education and experience.
Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
If this role is not for you
Please stay in touch and to see a complete list of our open career opportunities, please visit:
https://gladstone.org/careers
Hiring Range:
RA I $25-$29/hr.
RA II $66,560- $78,306K
Gladstone Perks & Benefits
People–work with talented, committed, and supportive teammates within an organization that values each member of its community.
A meaningful place to grow and learn–whether it’s your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone’s mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow’s leaders in an inspiring and diverse environment.
Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
Compensation–competitive salary. Title and salary will be commensurate with education and experience.
Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.
