Associate Engineer, High Throughput Operations



Berkeley, CA, USA
Posted on Thursday, July 11, 2024

Profluent is an AI-first protein design company. Founded in 2022, we develop deep generative models to design and validate novel, functional proteins to revolutionize biomedicine. Based in Berkeley, CA, we are backed by leading investors including Spark Capital, Insight Partners, Air Street Capital, AIX Ventures, and Convergent Ventures.

We are currently seeking a creative, passionate, and detail-oriented Associate Engineer to join the High Throughput Operations team. The focus of this role will be to develop and implement automated workflows for our in-house molecular cloning core, high throughput screening core, and NGS core. This candidate will work collaboratively with scientists, engineers, and bioinformaticians to develop a suite of assays for measuring gene editing activity and specificity.

This is an excellent opportunity to shape the future of AI-driven protein design and to work cross-functionally with a diverse team of experts across machine learning, protein engineering, cell biology, and gene editing.


  • Provide regular maintenance, calibration, and troubleshooting procedures on liquid handlers, high throughput lab equipment, and other integrated systems
  • Work cross-functionally to develop and improve high throughput processes for molecular biology, bacterial/mammalian cell screening, and NGS workflows
  • Write verification and validation test protocols and deliver reports including IQ/OQ/PQ
  • Generate SOPs and reports for validated automation processes and assist with implementation
  • Provide onsite operator support for automated workflows
  • Develop tools, reporting and operational documents for tracking equipment spare parts, service calls, and repairs
  • Build and maintain relationships with vendors and third-party service providers


  • BS/MS in science or engineering discipline with 0-2 years of relevant experience
  • Hands-on experience operating liquid handling systems (e.g. Tecan Fluent, Hamilton Star, Agilent Bravo, Beckman Coulter Biomek)
  • Hands-on experience with protocol development and operation of gene assembly, cloning, mammalian screening, and NGS workflows
  • Experience with high throughput assay development and validation
  • Can adapt to changing project priorities and timelines
  • Strong oral and written communication skills
  • Excellent analytical, critical thinking, and soft skills to build collaborative relationships that enable effective professional interactions with vendors and stakeholders

Preferences (but not required)

  • High level of familiarity with gene editing systems, genome-wide assays to measure gene editing activity, specificity, and DNA repair outcomes.
  • Experience working with external vendors and third-party service providers
  • Some software development experience using python, C#, C++ or other programming languages

Actual salary will be determined based on relevant skills, qualifications, experience, training, and market data. Benefits package may vary depending on company policies and eligibility criteria.

Hiring Salary Range
$99,800$122,000 USD

What we offer at Profluent

  • A high-growth opportunity with meaningful impact
  • Competitive compensation package
  • Health insurance (health/dental/vision)
  • Generous paid time off (PTO) policy
  • Commitment to physical and mental well-being
  • More benefits and perks to be added!

Profluent Bio, Inc is an equal opportunity employer promoting diversity and inclusion in the workspace. We do not discriminate on the basis of race, color, religion, marital status, age, national origin, ancestry, physical or mental disability, medical conditions, veteran status, sexual orientation, gender (including gender identity and gender expression), sex (which includes pregnancy, childbirth, and breastfeeding), genetic information, taking or requesting statutorily protected leave, or any other basis protected by law.

Legal authorization to work in the United States is required. In compliance with federal law, all persons hired will be required to verify identity and eligibility to work in the United States and to complete the required employment eligibility verification form upon hire.